Coding

Part:BBa_K3610007:Design

Designed by: Jonas Sebastian Trottmann   Group: iGEM20_UZurich   (2020-09-08)


EFR Ectodomain without signal sequence


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 82
    Illegal NheI site found at 1012
    Illegal NheI site found at 1930
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1598
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The juxtamembrane domain must be included in the sequence to ensure proper dimerization with the coreceptor. The signal sequence of the full length receptor was cleaved off out of frame.

The amino acid which in the full length version codes for the amino acid number 703 (Glu), which is at position 2035 in this part, has been changed as the part would otherwise have interferred with assembly standards. (GAA to GAG)

Source

Cyril Zipfel Lab at UZH, Switzerland

References

Macho, Alberto P.; Zipfel, Cyril (2014): Plant PRRs and the Activation of Innate Immune Signaling. In: Molecular Cell 54 (2), S. 263–272. DOI: 10.1016/j.molcel.2014.03.028.

Yan, Liming; Ma, Yuanyuan; Liu, Dan; Wei, Xiaochao; Sun, Yuna; Chen, Xiaoyue et al. (2012): Structural basis for the impact of phosphorylation on the activation of plant receptor-like kinase BAK1. In: Cell Res 22 (8), S. 1304–1308. DOI: 10.1038/cr.2012.74.

Pfeilmeier, Sebastian; George, Jeoffrey; Morel, Arry; Roy, Sonali; Smoker, Matthew; Stransfeld, Lena et al. (2019): Expression of the Arabidopsis thaliana immune receptor EFR in Medicago truncatula reduces infection by a root pathogenic bacterium, but not nitrogen-fixing rhizobial symbiosis. In: Plant biotechnology journal 17 (3), S. 569–579. DOI: 10.1111/pbi.12999.

Kunze, Gernot; Zipfel, Cyril; Robatzek, Silke; Niehaus, Karsten; Boller, Thomas; Felix, Georg (2004): The N terminus of bacterial elongation factor Tu elicits innate immunity in Arabidopsis plants. In: Plant Cell 16 (12), S. 3496–3507. DOI: 10.1105/tpc.104.026765.